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Tuan Bui - Research

The main goal of my research program is to understand how the nervous system controls movement, in particular, through the activity of the spinal cord. The lab seeks to characterize neurons involved in motor control, identify the circuits that they form and study their involvement in motor tasks such as locomotion (e.g. walking, swimming) and hand function. Experimental techniques include electrophysiology, immunocytochemistry, and computational modelling, and experimental models include zebrafish and mouse.  Below, I describe a few of the research themes in my lab.

LOCOMOTION

Locomotion is one of the most basic forms of movement. It is a rhythmic alternation of sets of muscles that support body weight and move the body in a particular direction. The spinal cord contains the necessary and sufficient neural machinery to allow animals to locomote, even in the absence of any inputs from the brain! This ability of vertebrate species to walk or swim is based upon the operations of an ensemble of neurons in the spinal cord which are wired together into a network. The activity of this network generates the rhythm that underlies walking and coordinates the muscles involved in locomotion. This network is also known as a Central Pattern Generator (CPG).


Left: A schematic of the lumbar spinal cord with recording electrodes attached to ventral roots (collection of motor nerves). Right: Electroneurograms during locomotion.

HAND CONTROL

If you've ever had an object slip through your hands, you are aware of how we can reflexively tighten our grip to secure that object.  A population of neurons in the spinal cord is known to play a crucial role in hand grasp that seems to be intimately tied to this ability to properly adjust grip force when the situation requires it.  These neurons, called dI3 interneurons, are a population of neurons found in the deep dorsal horn that are identified by their expression of the transcription factor Isl1.  dI3 interneurons receive sensory inputs originating from the skin and conveying touch.  In turn, these neurons send excitatory projections to motoneurons, the neurons that innervate muscles and directly control muscle contraction.  Silencing this spinal sensory-motor circuit led to striking deficits in hand grasp (Bui et al., 2013, Neuron).  Having identified the functional role of these neurons, we now hope to detail the fine mechanisms by which dI3 interneurons receive information from the skin conveying touch in order to control hand grip for everyday hand function. 

dI3 interneurons labelled with GFP
      dI3 INs labelled with GFP          dI3 INs in a traverse slice
                                                              of mouse spinal cord